High Five细胞
High Five（正式名称为BTI-Tn-5B1-4）是一种昆虫细胞系，起源于粉纹夜蛾的卵巢细胞[1]。 它是由美国的植物研究所BTI发现的。High Five 细胞是使用杆状病毒或转染，进行重组蛋白表达的其中一个最常用细胞系，并且比Sf9细胞等鳞翅类昆虫细胞系表达更多的重组蛋白[2][3][4]。它们可以在没有血清的情况下生长，并且可以以松散的附着状态或悬浊液培养[5] 。High Five细胞可产生大量的小分子核糖核酸、小分子干扰核糖核酸及piRNA，使其适合于研究以上三种类型的小沉默核糖核酸[6]。
参考资料
- ^ Wickham, TJ; Davis, T; Granados, RR; Shuler, ML; Wood, HA. Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.. Biotechnology progress. NaN, 8 (5): 391–6 [2019-11-23]. PMID 1369220. doi:10.1021/bp00017a003.
- ^ Davis, TR; Trotter, KM; Granados, RR; Wood, HA. Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation and secretion.. Bio/technology (Nature Publishing Company). 1992-10, 10 (10): 1148–50 [2019-11-23]. PMID 1368794. doi:10.1038/nbt1092-1148.
- ^ Wickham, TJ; Nemerow, GR. Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system.. Biotechnology progress. NaN, 9 (1): 25–30 [2019-11-23]. PMID 7764044. doi:10.1021/bp00019a004.
- ^ Granados RR, Guoxun L, Derksen AC, McKenna KA. A New Insect Cell Line from Trichoplusia ni (BTI-Tn-5B1-4) Susceptible to Trichoplusia ni Single Enveloped Nuclear Polyhedrosis. Virus. J. Invertebr. Pathol. 1994, 64: 260–266. doi:10.1016/s0022-2011(94)90400-6.
- ^ Invitrogen. Cell Lines. Growth and Maintenance of Insect cell lines (PDF). A.0. Thermo Fisher Scientific. 31 March 2015 [2015-11-09]. （原始内容存档 (PDF)于2016-03-04）.
|number=被忽略 (帮助) - ^ Fu, Yu; Yang, Yujing; Zhang, Han; Farley, Gwen; Wang, Junling; Quarles, Kaycee A; Weng, Zhiping; Zamore, Phillip D. The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology. eLife. 2018-01-29, 7 [2019-11-23]. ISSN 2050-084X. PMC 5844692
. doi:10.7554/eLife.31628. （原始内容存档于2019-11-23） （英语）.
外部链接
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