彗星實驗
彗星實驗（英語：comet assay），又稱作單細胞凝膠電泳實驗或彗星電泳法（英語：Single Cell Gel Electrophoresis assay，SCGE）是一種對單個細胞檢測DNA損傷的實驗技術。彗星實驗最初由Östling和Johansson在1984年發明，此後由 Singh等在1988年改進，此後成為了對細胞內DNA損傷與修復、遺傳毒性生物監測的最常用的標準實驗技術。步驟包括將細胞包覆在低融點的瓊脂糖中, 在中性或鹼性下(pH > 13)溶解細胞, 最後以凝膠電泳將包覆的已溶解細胞分離解析. 之所以稱為彗星, 是指細胞DNA在凝膠電泳解析分離後呈現的樣子有點像彗星.[1][2]
參考文獻
- ^ Tice, R. R. Single Cell Gel/Comet Assay: Guidelines for in vitro and in vivo Genetic Toxicology Testing. Environmental and Molecular Mutagenesis. 2000, 35 (3): 206–21. PMID 10737956. doi:10.1002/(SICI)10982280.
- ^ Nandhakumar S, Parasuraman S, Shanmugam M M, Rao K R, Chand P, Bhat B V. Evaluation of DNA damage using single-cell gel electrophoresis (Comet Assay).
延伸閱讀
- Dhawan & Anderson (2009): The Comet Assay in Toxicology. doi:10.1039/9781847559746
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- Rojas, E.; Lopez, M.C. & Valverde, M. (1999): Single cell gel electrophoresis: methodology and applications. Journal of Chromatography B 722(1-2): 225-254. doi:10.1016/S0378-4347(98)00313-2 (HTML abstract)
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