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Muse细胞

维基百科，自由的百科全书

Muse细胞（Multi-lineage differentiating stress enduring cell，多向分化压力耐受细胞）是由日本东北大学出泽真理课题组于2010年发现一种内源性、无成瘤性的多能干细胞[1]。几乎所有不同器官来源的结缔组织（如骨髓、外周血）中都有Muse细胞[2][3][4]。通常Muse细胞可从商业化的间充质细胞中分离到，如人成纤维细胞、骨髓间充质干细胞，以及脂肪源干细胞（adipose-derived stem cells）[5][6]。Muse细胞可自发分化或在细胞因子诱导作用下分化为三个胚层的细胞。Muse细胞的成瘤性较低，注入新的宿主体内也不会形成肿瘤，目前的观点认为这是因为该细胞的端粒酶活性较低[1]。Muse细胞同时被应用于小鼠骨骼肌退变、糖尿病性皮肤溃疡及急性重症肝炎等模型的修复研究中，研究发现Muse细胞在损伤组织中能够和宿主的组织顺利整合，并且分别向相应胚层的细胞分化[7][8][9]。有研究利用Muse细胞修复脑动脉堵塞后，引起局部缺血的中风小鼠模型，发现Muse细胞修现可以替代丢失的神经元，整合至梗死的皮质组织中，分化为神经元标志物表达阳性的细胞，并且有较为明显的功能修复[10]。

培养

当Muse细胞被放置到含有营养因子和细胞因子的培养基中进行培养时，超过百分之九十的细胞都会进行定向细胞分化。若该培养基含有肝细胞生长因子、成纤维细胞生长因子4和地塞米松的胰岛素-转铁蛋白-亚硒酸钠时，百分之九十以上的细胞会分化成肝细胞，并且在α-胎儿蛋白和人类白蛋白表达中呈现阳性反应。在骨或脂肪细胞的培养基中，百分之九十八以上的细胞会分化为骨钙蛋白或油红O阳性的细胞[11]。

参见

参考资料

  1. ^ 1.0 1.1 Kuroda, Y.; Kitada, M.; Wakao, S.; Nishikawa, K.; Tanimura, Y.; Makinoshima, H.; Goda, M.; Akashi, H.; Inutsuka, A.; Niwa, A.; Shigemoto, T.; Nabeshima, Y.; Nakahata, T.; Nabeshima, Y.-i.; Fujiyoshi, Y.; Dezawa, M. Unique multipotent cells in adult human mesenchymal cell populations. Proceedings of the National Academy of Sciences. 2010, 107 (19): 8639–43. Bibcode:2010PNAS..107.8639K. PMC 2889306 可免费查阅. PMID 20421459. doi:10.1073/pnas.0911647107. 
  2. ^ Wakao, S.; Kitada, M.; Kuroda, Y.; Shigemoto, T.; Matsuse, D.; Akashi, H.; Tanimura, Y.; Tsuchiyama, K.; Kikuchi, T.; Goda, M.; Nakahata, T.; Fujiyoshi, Y.; Dezawa, M. Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts. Proceedings of the National Academy of Sciences. 2011, 108 (24): 9875–80. Bibcode:2011PNAS..108.9875W. PMC 3116385 可免费查阅. PMID 21628574. doi:10.1073/pnas.1100816108. 
  3. ^ Dezawa, Mari. Muse Cells Provide the Pluripotency of Mesenchymal Stem Cells: Direct Contribution of Muse Cells to Tissue Regeneration. Cell Transplantation. 2016, 25 (5): 849–61. PMID 26884346. doi:10.3727/096368916X690881. 
  4. ^ Hori, Emiko; Hayakawa, Yumiko; Hayashi, Tomohide; Hori, Satoshi; Okamoto, Soushi; Shibata, Takashi; Kubo, Michiya; Horie, Yukio; Sasahara, Masakiyo; Kuroda, Satoshi. Mobilization of Pluripotent Multilineage-Differentiating Stress-Enduring Cells in Ischemic Stroke. Journal of Stroke and Cerebrovascular Diseases. 2016, 25 (6): 1473–81. PMID 27019988. doi:10.1016/j.jstrokecerebrovasdis.2015.12.033. 
  5. ^ Kuroda, Yasumasa; Wakao, Shohei; Kitada, Masaaki; Murakami, Toru; Nojima, Makoto; Dezawa, Mari. Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells. Nature Protocols. 2013, 8 (7): 1391–415. PMID 23787896. doi:10.1038/nprot.2013.076. 
  6. ^ Heneidi, Saleh; Simerman, Ariel A.; Keller, Erica; Singh, Prapti; Li, Xinmin; Dumesic, Daniel A.; Chazenbalk, Gregorio. Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue. PLoS ONE. 2013, 8 (6): e64752. Bibcode:2013PLoSO...864752H. PMC 3673968 可免费查阅. PMID 23755141. doi:10.1371/journal.pone.0064752. 
  7. ^ Simerman, AA; Dumesic, DA; Chazenbalk, GD. Pluripotent muse cells derived from human adipose tissue: a new perspective on regenerative medicine and cell therapy.. Clinical and translational medicine. 2014, 3: 12 [2019-11-22]. PMID 24940477. doi:10.1186/2001-1326-3-12. 
  8. ^ Wakao, S; Akashi, H; Kushida, Y; Dezawa, M. Muse cells, newly found non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues.. Pathology international. 2014-01, 64 (1): 1–9 [2019-11-22]. PMID 24471964. doi:10.1111/pin.12129. 
  9. ^ Kuroda, Y; Dezawa, M. Mesenchymal stem cells and their subpopulation, pluripotent muse cells, in basic research and regenerative medicine.. Anatomical record (Hoboken, N.J. : 2007). 2014-01, 297 (1): 98–110 [2019-11-22]. PMID 24293378. doi:10.1002/ar.22798. 
  10. ^ Yamauchi, T; Kuroda, Y; Morita, T; Shichinohe, H; Houkin, K; Dezawa, M; Kuroda, S. Therapeutic effects of human multilineage-differentiating stress enduring (MUSE) cell transplantation into infarct brain of mice.. PloS one. 2015, 10 (3): e0116009 [2019-11-22]. PMID 25747577. doi:10.1371/journal.pone.0116009. 
  11. ^ Ogura, Fumitaka; Wakao, Shohei; Kuroda, Yasumasa; Tsuchiyama, Kenichiro; Bagheri, Mozhdeh; Heneidi, Saleh; Chazenbalk, Gregorio; Aiba, Setsuya; Dezawa, Mari. Human Adipose Tissue Possesses a Unique Population of Pluripotent Stem Cells with Nontumorigenic and Low Telomerase Activities: Potential Implications in Regenerative Medicine. Stem Cells and Development. 2014, 23 (7): 717–28. PMID 24256547. doi:10.1089/scd.2013.0473. 
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