COS细胞

COS是衍生自猴肾组织的成纤维细胞样细胞系，常用于科研用途。

特征

COS细胞是通过用能产生大T抗原^[1]的猿猴空泡病毒40 （SV40）永生化（immortalizing）CV-1细胞而获得的^[2] ，而CV-1细胞则来自非洲绿猴的肾脏^[3] 。首字母缩略词是“COS”，因为COS细胞源自于CV-1细胞，并且携带着SV40的遗传物质^[1] 。COS细胞常用的两种形式是COS-1细胞和COS-7细胞。COS-1细胞及COS-7细胞都是经带有编码野生型T抗原的起始失活突变的SV40转染得到^[4]^[5]，其中COS-1细胞含有一个来自SV40基因组全部早期区域的组成型拷贝。

科研用途

生物学家在研究SV40时，经常使用COS细胞系。来自这些品系的细胞也经常被转染，以产生用于分子生物学、生物化学和细胞生物学实验的重组蛋白^[6] 。当将具有SV40启动子的表现载体（英语：Expression vector），引入COS细胞时，该载体可以被大T抗原复制。这些COS细胞经过基因修饰，从其自身的基因组中产生T抗原^[6]^[7]。

另见

COS-7细胞
猿猴空泡病毒40

参考资料

^ ^1.0 ^1.1 Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. January 1981, 23 (1): 175–82. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.
^ Jensen FC, Girardi AJ, Gilden RV, Koprowski H. Infection of human and simian tissue cultures with rous sarcoma virus. Proc. Natl. Acad. Sci. U.S.A. July 1964, 52 (1): 53–9. Bibcode:1964PNAS...52...53J. PMC 300571 . PMID 14192657. doi:10.1073/pnas.52.1.53.
^ Fanelli, Alex. COS-7 cell line: Origin and Characteristics. 2016 [28 November 2017]. （原始内容存档于2019-12-22）.
^ Gluzman, Y. SV40-transformed simian cells support the replication of early SV40 mutants.. Cell. 1981-01, 23 (1): 175–82 [2019-12-08]. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.
^ Mellon, P; Parker, V; Gluzman, Y; Maniatis, T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.. Cell. 1981-12, 27 (2 Pt 1): 279–88 [2019-12-08]. PMID 6277501. doi:10.1016/0092-8674(81)90411-6.
^ ^6.0 ^6.1 Aruffo, A. Transient expression of proteins using COS cells. Curr Protoc Mol Biol. November 2002,. Chapter 16: Unit 16.12. ISBN 0471142727. PMID 18265298. doi:10.1002/0471142727.mb1612s60.
^ WEINANDER, Rolf; et al. Heterologous expression of rat liver microsomal glutathione transferase in simian COS cells and Escherichia coli. Biochem. J. 1995, 311 (3): 861–866. PMC 1136080 . PMID 7487942. doi:10.1042/bj3110861.

外部链接

Cellosaurus entry for COS-1（页面存档备份，存于互联网档案馆）
Cellosaurus entry for COS-7（页面存档备份，存于互联网档案馆）

[Gluzman_1981-1] 1.0 ^1.1 Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. January 1981, 23 (1): 175–82. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.

[pmid14192657-2] Jensen FC, Girardi AJ, Gilden RV, Koprowski H. Infection of human and simian tissue cultures with rous sarcoma virus. Proc. Natl. Acad. Sci. U.S.A. July 1964, 52 (1): 53–9. Bibcode:1964PNAS...52...53J. PMC 300571 . PMID 14192657. doi:10.1073/pnas.52.1.53.

[3] Fanelli, Alex. COS-7 cell line: Origin and Characteristics. 2016 [28 November 2017]. （原始内容存档于2019-12-22）.

[a-4] Gluzman, Y. SV40-transformed simian cells support the replication of early SV40 mutants.. Cell. 1981-01, 23 (1): 175–82 [2019-12-08]. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.

[b-5] Mellon, P; Parker, V; Gluzman, Y; Maniatis, T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.. Cell. 1981-12, 27 (2 Pt 1): 279–88 [2019-12-08]. PMID 6277501. doi:10.1016/0092-8674(81)90411-6.

[Aruffo-6] 6.0 ^6.1 Aruffo, A. Transient expression of proteins using COS cells. Curr Protoc Mol Biol. November 2002,. Chapter 16: Unit 16.12. ISBN 0471142727. PMID 18265298. doi:10.1002/0471142727.mb1612s60.

[7] WEINANDER, Rolf; et al. Heterologous expression of rat liver microsomal glutathione transferase in simian COS cells and Escherichia coli. Biochem. J. 1995, 311 (3): 861–866. PMC 1136080 . PMID 7487942. doi:10.1042/bj3110861.

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