UBTF

Upstream binding transcription factor, RNA polymerase I
RNA聚合酶I上游结合因子
Upstream binding transcription factor, RNA polymerase I; RNA聚合酶I上游结合因子
	PDB rendering based on 1k99.
有效结构
PDB	直系同源检索：PDBe, RCSB
PDB查询代码列表
	1K99, 1L8Y, 1L8Z, 2HDZ
标识
代号	UBTF; NOR-90; UBF; UBF-1; UBF1
扩展标识	遗传学：600673 鼠基因：98512 同源基因：7970 GeneCards: UBTF Gene
基因本体论描述
分子功能	· DNA binding; · protein binding;
细胞成分	· nucleoplasm; · nucleolus; · cytoplasm;
生物过程	· blastocyst growth; · regulation of transcription from RNA polymerase I promoter; · transcription from RNA polymerase I promoter; · transcription initiation from RNA polymerase I promoter; · transcription elongation from RNA polymerase I promoter; · termination of RNA polymerase I transcription; · embryo implantation; · gene expression; · positive regulation of transcription from RNA polymerase I promoter;
	Sources: Amigo / QuickGO
RNA表达模式
	更多表达数据
直系同源体
物种	人类
Entrez	7343
Ensembl	ENSG00000108312
UniProt	P17480
mRNA序列	NM_001076683
蛋白序列	NP_001070151
基因位置	Chr 17:; 42.28 – 42.3 Mb
PubMed查询	[1]
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核仁转录因子1（Nucleolar transcription factor 1）又叫上游结合转录因子（Upstream binding transcription factor，缩写UBTF）或上游结合因子1（Upstream binding factor 1，缩写UBF1）是一种由人类基因UBTF 所编码的蛋白质^[1]^[2]。

功能

UBTF是18S、5.8S和28S的核糖体RNA的转录所必须的转录因子，和SL1因子（一个四聚体，包括一个TATA结合蛋白和3个转录辅助因子TAF1（英语：TAF1））共同起作用。在人体中存在94和97kD的两种UBF多肽链。UBF是一种核仁磷酸化蛋白（nucleolar phosphoprotein），同时具有DNA结合结构域和反式激活结构域，通过HMG盒结合到人rRNA启动子的核心与上游部位。^[2]

相互作用

UBTF已知能与酪蛋白激酶2α亚基（英语：Casein kinase 2, alpha 1）^[3]、TAF1C^[4]^[5]、视网膜母细胞瘤蛋白^[6]和TAF1^[7]进行交互作用。

参考文献

^ Matera AG, Wu W, Imai H, O'Keefe CL, Chan EK. Molecular cloning of the RNA polymerase I transcription factor hUBF/NOR-90 (UBTF) gene and localization to 17q21.3 by fluorescence in situ hybridization and radiation hybrid mapping. Genomics. May 1997, 41 (1): 135–8. PMID 9126496. doi:10.1006/geno.1997.4647.
^ ^2.0 ^2.1 Entrez Gene: UBTF upstream binding transcription factor, RNA polymerase I.
^ Voit, R; Kuhn A, Sander E E, Grummt I. Activation of mammalian ribosomal gene transcription requires phosphorylation of the nucleolar transcription factor UBF. Nucleic Acids Res. (ENGLAND). July 1995, 23 (14): 2593–9. ISSN 0305-1048. PMC 307079 . PMID 7651819. doi:10.1093/nar/23.14.2593.
^ Voit, R; Grummt I. Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription. Proc. Natl. Acad. Sci. U.S.A. (United States). November 2001, 98 (24): 13631–6. ISSN 0027-8424. PMC 61092 . PMID 11698641. doi:10.1073/pnas.231071698.
^ Zhai, W; Comai L. Repression of RNA Polymerase I Transcription by the Tumor Suppressor p53. Mol. Cell. Biol. (UNITED STATES). August 2000, 20 (16): 5930–8. ISSN 0270-7306. PMC 86070 . PMID 10913176. doi:10.1128/MCB.20.16.5930-5938.2000.
^ Hannan, K M; Hannan R D, Smith S D, Jefferson L S, Lun M, Rothblum L I. Rb and p130 regulate RNA polymerase I transcription: Rb disrupts the interaction between UBF and SL-1. Oncogene (ENGLAND). October 2000, 19 (43): 4988–99. ISSN 0950-9232. PMID 11042686. doi:10.1038/sj.onc.1203875.
^ Lin, Chih-Yin; Tuan JoAnn, Scalia Pierluigi, Bui Tiffany, Comai Lucio. The cell cycle regulatory factor TAF1 stimulates ribosomal DNA transcription by binding to the activator UBF. Curr. Biol. (England). Dec 2002, 12 (24): 2142–6. ISSN 0960-9822. PMID 12498690. doi:10.1016/S0960-9822(02)01389-1.

延伸阅读

Chan EK, Imai H, Hamel JC, Tan EM. Human autoantibody to RNA polymerase I transcription factor hUBF. Molecular identity of nucleolus organizer region autoantigen NOR-90 and ribosomal RNA transcription upstream binding factor. J. Exp. Med. 1991, 174 (5): 1239–44. PMC 2119007 . PMID 1940801. doi:10.1084/jem.174.5.1239.
Jantzen HM, Admon A, Bell SP, Tjian R. Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins. Nature. 1990, 344 (6269): 830–6. PMID 2330041. doi:10.1038/344830a0.
Bell SP, Learned RM, Jantzen HM, Tjian R. Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis. Science. 1988, 241 (4870): 1192–7. PMID 3413483. doi:10.1126/science.3413483.
Voit R, Kuhn A, Sander EE, Grummt I. Activation of mammalian ribosomal gene transcription requires phosphorylation of the nucleolar transcription factor UBF. Nucleic Acids Res. 1995, 23 (14): 2593–9. PMC 307079 . PMID 7651819. doi:10.1093/nar/23.14.2593.
Hempel WM; Cavanaugh AH; Hannan RD; et al. The species-specific RNA polymerase I transcription factor SL-1 binds to upstream binding factor. Mol. Cell. Biol. 1996, 16 (2): 557–63. PMC 231034 . PMID 8552083.
Hanada K; Song CZ; Yamamoto K; et al. RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription. EMBO J. 1996, 15 (9): 2217–26. PMC 450146 . PMID 8641287.
Pluta AF, Earnshaw WC. Specific interaction between human kinetochore protein CENP-C and a nucleolar transcriptional regulator. J. Biol. Chem. 1996, 271 (31): 18767–74. PMID 8702533. doi:10.1074/jbc.271.31.18767.
Whitehead CM, Winkfein RJ, Fritzler MJ, Rattner JB. ASE-1: a novel protein of the fibrillar centres of the nucleolus and nucleolus organizer region of mitotic chromosomes. Chromosoma. 1998, 106 (8): 493–502. PMID 9426281. doi:10.1007/s004120050271.
Voit R, Hoffmann M, Grummt I. Phosphorylation by G1-specific cdk-cyclin complexes activates the nucleolar transcription factor UBF. EMBO J. 1999, 18 (7): 1891–9. PMC 1171274 . PMID 10202152. doi:10.1093/emboj/18.7.1891.
Liu CJ, Wang H, Lengyel P. The interferon-inducible nucleolar p204 protein binds the ribosomal RNA-specific UBF1 transcription factor and inhibits ribosomal RNA transcription. EMBO J. 1999, 18 (10): 2845–54. PMC 1171365 . PMID 10329630. doi:10.1093/emboj/18.10.2845.
Hannan KM; Hannan RD; Smith SD; et al. Rb and p130 regulate RNA polymerase I transcription: Rb disrupts the interaction between UBF and SL-1. Oncogene. 2000, 19 (43): 4988–99. PMID 11042686. doi:10.1038/sj.onc.1203875.
Kalousek I, Krízková P. Lymphocyte mitogenic transformation is accompanied by phosphorylation of the nucleolar transcription factor UBF. Cell. Mol. Biol. (Noisy-le-grand). 2001, 46 (7): 1163–71. PMID 11075946.
Pelletier G; Stefanovsky VY; Faubladier M; et al. Competitive recruitment of CBP and Rb-HDAC regulates UBF acetylation and ribosomal transcription. Mol. Cell. 2002, 6 (5): 1059–66. PMID 11106745. doi:10.1016/S1097-2765(00)00104-0.
Miller G; Panov KI; Friedrich JK; et al. hRRN3 is essential in the SL1-mediated recruitment of RNA Polymerase I to rRNA gene promoters. EMBO J. 2001, 20 (6): 1373–82. PMC 145519 . PMID 11250903. doi:10.1093/emboj/20.6.1373.
Seither P, Iben S, Thiry M, Grummt I. PAF67, a novel protein that is associated with the initiation-competent form of RNA polymerase I. Biol. Chem. 2002, 382 (8): 1163–70. PMID 11592397. doi:10.1515/BC.2001.146.
Voit R, Grummt I. Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription. Proc. Natl. Acad. Sci. U.S.A. 2002, 98 (24): 13631–6. PMC 61092 . PMID 11698641. doi:10.1073/pnas.231071698.
Stefanovsky VY; Pelletier G; Hannan R; et al. An immediate response of ribosomal transcription to growth factor stimulation in mammals is mediated by ERK phosphorylation of UBF. Mol. Cell. 2002, 8 (5): 1063–73. PMID 11741541. doi:10.1016/S1097-2765(01)00384-7.
Andersen JS; Lyon CE; Fox AH; et al. Directed proteomic analysis of the human nucleolus. Curr. Biol. 2002, 12 (1): 1–11. PMID 11790298. doi:10.1016/S0960-9822(01)00650-9.
Dühr S; Torres-Montaner A; Astola A; et al. Molecular analysis of the 5' region of human ribosomal transcription factor UBF. DNA Seq. 2002, 12 (4): 267–72. PMID 11916260. doi:10.3109/10425170109025001.

[pmid9126496-1] Matera AG, Wu W, Imai H, O'Keefe CL, Chan EK. Molecular cloning of the RNA polymerase I transcription factor hUBF/NOR-90 (UBTF) gene and localization to 17q21.3 by fluorescence in situ hybridization and radiation hybrid mapping. Genomics. May 1997, 41 (1): 135–8. PMID 9126496. doi:10.1006/geno.1997.4647.

[entrez-2] 2.0 ^2.1 Entrez Gene: UBTF upstream binding transcription factor, RNA polymerase I.

[pmid7651819-3] Voit, R; Kuhn A, Sander E E, Grummt I. Activation of mammalian ribosomal gene transcription requires phosphorylation of the nucleolar transcription factor UBF. Nucleic Acids Res. (ENGLAND). July 1995, 23 (14): 2593–9. ISSN 0305-1048. PMC 307079 . PMID 7651819. doi:10.1093/nar/23.14.2593.

[pmid11698641-4] Voit, R; Grummt I. Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription. Proc. Natl. Acad. Sci. U.S.A. (United States). November 2001, 98 (24): 13631–6. ISSN 0027-8424. PMC 61092 . PMID 11698641. doi:10.1073/pnas.231071698.

[pmid10913176-5] Zhai, W; Comai L. Repression of RNA Polymerase I Transcription by the Tumor Suppressor p53. Mol. Cell. Biol. (UNITED STATES). August 2000, 20 (16): 5930–8. ISSN 0270-7306. PMC 86070 . PMID 10913176. doi:10.1128/MCB.20.16.5930-5938.2000.

[pmid11042686-6] Hannan, K M; Hannan R D, Smith S D, Jefferson L S, Lun M, Rothblum L I. Rb and p130 regulate RNA polymerase I transcription: Rb disrupts the interaction between UBF and SL-1. Oncogene (ENGLAND). October 2000, 19 (43): 4988–99. ISSN 0950-9232. PMID 11042686. doi:10.1038/sj.onc.1203875.

[pmid12498690-7] Lin, Chih-Yin; Tuan JoAnn, Scalia Pierluigi, Bui Tiffany, Comai Lucio. The cell cycle regulatory factor TAF1 stimulates ribosomal DNA transcription by binding to the activator UBF. Curr. Biol. (England). Dec 2002, 12 (24): 2142–6. ISSN 0960-9822. PMID 12498690. doi:10.1016/S0960-9822(02)01389-1.

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