降解体
降解体（Degradosome）是存在于大多数细菌中的一种多亚基蛋白质复合物，其功能为参与核糖体RNA的加工以及信使RNA的降解，[1][2]故又可称为RNA降解体。
发现
1994年，瑞士日内瓦大学的Henry Krisch所领导的实验室和英国剑桥大学的Christopher Higgins所领导的实验室分别发表文章提出了细菌中存在着一种可以降解RNA的蛋白质复合物，也就是降解体；[3][4][5]他们是在对大肠杆菌的RNase E和PNPase的研究和鉴定过程中得到这一发现。[1]
组成
降解体的主要成分为核糖核酸酶E（RNase E）、多核苷酸磷酸化酶（PNPase）、RNase螺旋酶B（RhlB）和烯醇酶（enolase）。[6][7]
参考文献
- ^ 1.0 1.1 （英文）Carpousis AJ. The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E. Annu Rev Microbiol. 2007, 61: 71–87. PMID 17447862.
- ^ （英文）Carpousis AJ. The Escherichia coli RNA degradosome: structure, function and relationship in other ribonucleolytic multienzyme complexes. Biochem Soc Trans. 2002, 30: 150–155. PMID 12035760.
- ^ （英文）Carpousis AJ, Van Houwe G, Ehretsmann C, Krisch HM. Copurification of E. coli RNase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation. Cell. 1994, 76: 889–900.
- ^ （英文）Causton H, Py B, McLaren RS, Higgins CF. mRNA degradation in Escherichia coli: a novel factor which impedes the exoribonucleolytic activity of PNPase at stem-loop structures. Mol Microbiol. 1994, 14: 731–741.
- ^ （英文）Py B, Causton H, Mudd EA, Higgins CF. A protein complex mediating mRNA degradation in Escherichia coli. Mol Microbiol. 1994, 14: 717–729.
- ^ （英文）Miczak A, Kaberdin VR, Wei CL, Lin-Chao S. Proteins associated with RNase E in a multicomponent ribonucleolytic complex. Proc. Natl. Acad. Sci. USA. 1996, 93: 3865–3869.
- ^ （英文）Py B, Higgins CF, Krisch HM, Carpousis AJ. A DEAD-box RNA helicase in the Escherichia coli RNA degradosome. Nature. 1996, 381: 169–172.