DLD-1

DLD-1是非整数倍体的人类结直肠腺癌细胞系^[1] (Human Dukes’s type C colorectal adenocarcinoma cell line) ，最初是于1977年至1979年间分离出来的两株结直肠腺癌细胞株之一，对p53蛋白及多种癌基因 (例如C-Myc^[2]、KRAS（英语：KRAS）^[3]、HRAS（英语：HRAS）及NRAS（英语：Neuroblastoma RAS viral oncogene homolog）等^[4]) 呈现阳性反应。有研究指出其拥有较强的爬行和侵袭能力，故而拥有高迁移性与侵袭能力^[5]。

科研用途

2006年，有科研人员发现生育三烯酚（英语：Tocotrienol）以时间和剂量依赖性方式抑制DLD-1细胞的端粒酶活性，其中δ-生育三烯酚抑制其活性的效果最明显，表明生育三烯酚充当端粒酶的有效候选调节剂^[6]。2009年，有学者研究不同浓度的肝素对DLD-1细胞的影响，因为之前有硏究指出肝素对某些癌症的凋亡和抗增殖作用，然而研究结果最终表明肝素在体外对结肠癌细胞没有明显的抗增殖和凋亡作用^[7]。

参考资料

^ Chen, TR; Dorotinsky, CS; McGuire, LJ; Macy, ML; Hay, RJ. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin.. Cancer genetics and cytogenetics. 1995-06, 81 (2): 103–8 [2019-12-20]. PMID 7621404. doi:10.1016/0165-4608(94)00225-z. （原始内容存档于2019-12-20）.
^ Okuyama, H.; Endo, H.; Akashika, T.; Kato, K.; Inoue, M. Downregulation of c-MYC Protein Levels Contributes to Cancer Cell Survival under Dual Deficiency of Oxygen and Glucose. Cancer Research. 2010-10-27, 70 (24): 10213–10223. doi:10.1158/0008-5472.CAN-10-2720.
^ Dou, Y; Cha, DJ; Franklin, JL; Higginbotham, JN; Jeppesen, DK; Weaver, AM; Prasad, N; Levy, S; Coffey, RJ; Patton, JG; Zhang, B. Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes.. Scientific reports. 2016-11-28, 6: 37982 [2019-12-20]. PMID 27892494. doi:10.1038/srep37982. （原始内容存档于2019-12-20）.
^ Grabocka, E; Pylayeva-Gupta, Y; Jones, MJ; Lubkov, V; Yemanaberhan, E; Taylor, L; Jeng, HH; Bar-Sagi, D. Wild-type H- and N-Ras promote mutant K-Ras-driven tumorigenesis by modulating the DNA damage response.. Cancer cell. 2014-02-10, 25 (2): 243–56 [2019-12-20]. PMID 24525237. doi:10.1016/j.ccr.2014.01.005.
^ 林建贤.探讨胆囊收缩素受体A在人类大肠癌细胞的表现量以及与细胞移动和侵袭能力的关联性.中山医学大学生化暨生物科技研究所学位论文.2016年
^ Eitsuka, T; Nakagawa, K; Miyazawa, T. Down-regulation of telomerase activity in DLD-1 human colorectal adenocarcinoma cells by tocotrienol.. Biochemical and biophysical research communications. 2006-09-15, 348 (1): 170–5 [2019-12-20]. PMID 16875674. doi:10.1016/j.bbrc.2006.07.029. （原始内容存档于2019-12-20）.
^ Uzun, Y; Akdogan, E; Ozdemir, F; Ovali, E. The effects of heparin on DLD-1 colon cancer cell line.. Bratislavske lekarske listy. 2009, 110 (1): 3–6 [2019-12-20]. PMID 19408822. （原始内容存档于2019-12-20）.

外部链接

Cellosaurus entry for DLD-1（页面存档备份，存于互联网档案馆）

[1] Chen, TR; Dorotinsky, CS; McGuire, LJ; Macy, ML; Hay, RJ. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin.. Cancer genetics and cytogenetics. 1995-06, 81 (2): 103–8 [2019-12-20]. PMID 7621404. doi:10.1016/0165-4608(94)00225-z. （原始内容存档于2019-12-20）.

[2] Okuyama, H.; Endo, H.; Akashika, T.; Kato, K.; Inoue, M. Downregulation of c-MYC Protein Levels Contributes to Cancer Cell Survival under Dual Deficiency of Oxygen and Glucose. Cancer Research. 2010-10-27, 70 (24): 10213–10223. doi:10.1158/0008-5472.CAN-10-2720.

[3] Dou, Y; Cha, DJ; Franklin, JL; Higginbotham, JN; Jeppesen, DK; Weaver, AM; Prasad, N; Levy, S; Coffey, RJ; Patton, JG; Zhang, B. Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes.. Scientific reports. 2016-11-28, 6: 37982 [2019-12-20]. PMID 27892494. doi:10.1038/srep37982. （原始内容存档于2019-12-20）.

[4] Grabocka, E; Pylayeva-Gupta, Y; Jones, MJ; Lubkov, V; Yemanaberhan, E; Taylor, L; Jeng, HH; Bar-Sagi, D. Wild-type H- and N-Ras promote mutant K-Ras-driven tumorigenesis by modulating the DNA damage response.. Cancer cell. 2014-02-10, 25 (2): 243–56 [2019-12-20]. PMID 24525237. doi:10.1016/j.ccr.2014.01.005.

[5] 林建贤.探讨胆囊收缩素受体A在人类大肠癌细胞的表现量以及与细胞移动和侵袭能力的关联性.中山医学大学生化暨生物科技研究所学位论文.2016年

[6] Eitsuka, T; Nakagawa, K; Miyazawa, T. Down-regulation of telomerase activity in DLD-1 human colorectal adenocarcinoma cells by tocotrienol.. Biochemical and biophysical research communications. 2006-09-15, 348 (1): 170–5 [2019-12-20]. PMID 16875674. doi:10.1016/j.bbrc.2006.07.029. （原始内容存档于2019-12-20）.

[7] Uzun, Y; Akdogan, E; Ozdemir, F; Ovali, E. The effects of heparin on DLD-1 colon cancer cell line.. Bratislavske lekarske listy. 2009, 110 (1): 3–6 [2019-12-20]. PMID 19408822. （原始内容存档于2019-12-20）.

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